The perineum is defined as the area located:

The perineum is anatomically defined as the region that is located between the vagina and the anus in females, and between the scrotum and anus in males. This area is significant in both anatomy and medical contexts, as it encompasses several important structures and is subject to various medical assessments and procedures. Understanding this region helps in recognizing its role in childbirth, sexual function, and potential health issues. The other options refer to specific structures related to female anatomy but do not encompass the full definition and boundaries of the perineum. Thus, identifying it correctly as the area between the vagina and anus clarifies its anatomical significance.